Abstract
Summary
Activation of growth factor for cultures of Caenorhabditis briggsae is accompanied by formation of a fine, flocculent precipitate which can be measured by increased absorbance at 550 mμ. Biological activity resides in the precipitate after separation by sedimentation. By precipitation the growth factor is made available to the nematode in a concentrated and particulate form. If the level of soluble protein in the medium is insufficient, concentration may be essential for growth. The particulate form may have a specific physiological role for bacteria-feeding nematodes. Other proteins do not support growth of C. briggsae even when used at high levels or subjected to activation procedures.
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