Abstract
Summary
A baby hamster kidney cell line transformed in vitro by adenovirus type 12 produced high titer T antigen. Using different tumor-bearing hamster sera in the indirect fluorescent antibody technic the resultant staining was either confined to the cytoplasm or was present also in the nucleus. Both the intranuclear T antigen and intracytoplasmic T antigen when isolated behaved similarly on hydroxylapatite columns and on starch block electrophoresis. On hydroxylapatite columns the majority of the recovered CF activity was eluted with 0.2 M salt. On starch block electrophoresis all of the recovered CF activity was present in one peak effecting a 10-fold increase in specific activity by a one-step procedure. Preparations with highest specific activity were obtained from fractions of nuclear preparations separated by starch block electrophoresis.
The excellent technical assistance of Mrs. Jessie Doleman, Miss Shirley J. Hagens, Mrs. Beatrice R. O'Keefe and Mrs. Inta Ziedins is gratefully acknowledged.
Get full access to this article
View all access options for this article.