Abstract
Summary
Infection of adult human skin organ cultures with HF herpes simplex virus (type 2) resulted in appearance of new virus in the culture medium in 3 or 4 days. This was associated with epidermal cell cytopa-thogenesis which resembled in vivo herpes simplex infections. Cultures consisted of 4-mm punches of split-thickness skin incubated in 1 ml of medium in 16 × 125-mm screw-capped roller tubes at 35°. Cultures were easy to prepare in numbers, manipulate, and maintain; and viral reproduction and cytopathogenesis were reproducible.
Get full access to this article
View all access options for this article.