Abstract
Summary
We found that the entire array of enzymes in Pseudomonas perjectomarinus that account for reduction of nitrate to nitrogen, including that which reduces nitrous oxide to nitrogen, was synthesized in 40, but not 35, min of incubation in a complete medium containing nitrate. Nitrite accumulated during the first 5-6 hr of culture despite the fact that we could demonstrate enzymes in crude cell-free extracts that reduced nitrite, nitric oxide, and nitrous oxide. To explain this, we found that nitrate suppressed the activity of nitric oxide reducing enzyme in crude extracts, as well as that in a fraction containing none of the other relevant reducing systems.
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