Correlation Between Propagation of a Bovine Enterovirus and Development of Receptor-Like Material in Tissue Mince 1

Abstract

Summary

Bovine enterovirus (BE-1) replicates well in primary cell cultures of calf kidney within 24 hr after inoculation. Debris (2.5-20%) prepared from such cell cultures inactivates greater than 90% of the infectivi-ty of a BE-1 inoculum. Freshly excised calf kidney will support the replication of BE-1 within 24 hr only if incubated in vitro for 24 hr or longer prior to virus inoculation. Correlated with the development of sensitivity to virus infection, is the appearance of virus inactivating materials (receptors) in homogenates prepared from the incubated mince. The development of virus sensitivity and receptor material presumably was not due to the growth of new cells, since similar events occurred when cell division was inhibited by the incorporation of colchicine into the maintenance medium.