Abstract
Summary
Immunodiffusion of bile against specific antisera revealed 17 serum proteins in 10 normal gallbladder bile samples. Seven were common to all 10 samples. Five others were present in at least 50% of the biles. Analytical disc electrophoresis gave a mean of 12 bands staining with amido-schwarz with a range of 8-20. Because of the variability of bile patterns no uniform DEP pattern for normal gallbladder bile could be established, although relative relationships of major bands usually were recognizable. By use of DEP-immunodiffusion it was possible to identify and locate 12 specific serum proteins in the polyacrylamide electrophoretic patterns of bile.
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