Abstract
Summary
A plaque assay for rubella virus was developed using hemadsorption of pigeon erythrocytes to demonstrate foci of infected cells. Tests conducted by this method can be completed more rapidly than previously-described plaquing procedures based upon a cytopathic effect of the virus, and they are not beset with the variables inherent in interference technics. The number of plaques demonstrable by hemadsorption was proportional to the concentration of virus used to infect the cells, indicating that each plaque was produced by a single infectious particle. Infectivity titers of rubella preparations obtained by plaque assays were comparable to those obtained by the interference technic. Rubella antibody could be assayed by a plaque reduction technic, and plaque production could be used to detect small amounts of virus at low passage levels of field strains of rubella virus.
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