Abstract
Summary
An organ culture method for studying the migration of connective tissue cells into wounded tissues was described. Cells at the periphery of a frozen, killed zone appeared to undergo a period of mobilization during the first 48 hr after injury. After mobilization, cells began to migrate into the killed zone, gradually but only sparsely refilling the previously acellular area by 96 hr after injury. Colchicine (1 × 10-7 M/liter) totally inhibited cell movement when applied either before or after cell movement had already begun. The inhibitory effects of colchicine were completely reversible. No mitotic figures were observed at any time period studied (0-96 hr).
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