Abstract
Summary
Sixteen- and 19-day-old embryonic chick duodenum can be cultured for at least 3 days in a chemically defined serum-free medium.
No significant morphologic difference is observed whether or not serum is added to the medium.
Cells in mitosis are present in all cultures and the distribution of mitoses indicates mat uration of the tissue in culture with or without serum.
Invertase activity is increased in cultures without serum and the induction of invertase by hydrocortisone is greater in tissues cultured in serum-free medium.
The utilization of glucose by the duodenum in organ culture is decreased in the presence of hydrocortisone.
The utilization or production of the individual amino acids by the chick embryo duodenum in organ culture in the presence and absence of hydrocortisone has been described.
Get full access to this article
View all access options for this article.