Abstract
Summary
Lipid synthesis in rat epiphyseal cartilage has been studied in relation to normal growth and the production of rickets. Cartilage was incubated in vitro in modified Krebs-Ringer bicarbonate buffer with added I-14C palmitic acid. In normal 28-day-old male weanling rats, the total uptake of 1-14C palmitic acid from the media showed a gradual increase with time. Fractionation of the lipids showed 64.6% of the label in free fatty acids; 19.2% in triglycerides; 12.8% in phospholipids; and 6.2% in cholesterol and cholesterol esters. In rachitic rats of comparable age, the total uptake of 1-14C palmitic acid was increased significantly, indicating an increased rate of lipid synthesis.
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