Biosynthesis of Amino Acids from 14 C-U Glucose,Pyruvate,and Acetate by Erythrocytic Forms of P. knowlesi,in Vitro ∗

Abstract

Summary

Erythrocytic forms of P. knowlesi were grown for one or part of one schizogonic cycle in a medium containing ¹⁴C-U glucose, pyruvate, or acetate. Radioactive measurement of the amino acids of digested parasitic protein revealed ¹⁴C in aspartic acid, glutamic acid, and alanine. Both plasmodia and leukocytes were found to synthesize the three amino acids from the used precursors. No radioactivity was found in threonine, serine, glycine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine, and arginine.