Abstract
Summary
p-Nitrobluetetrazolium reductases in Mycobacterium tuberculosis were examined by a rapid electrophoretic technique. This procedure not only allowed the demonstration that total diaphorase activity was the resultant effect of multiple enzymes but was found to be adaptable to the study of individual tetrazolium reductases. In this manner, Triton X-100 was shown to have an overall stimulatory effect for all reductases, while the effect of menadione was most pronounced for a single reductase. Menadione sodium bisulfite not only could not replace menadione for full activity but was shown to be inhibitory for specific reductases. Most of the reductases were unable to utilize NADPH for the reduction of p-nitrobluetetrazolium.
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