Abstract
Summary
An adaptation of the enzyme-conjugated antibody technique for detection of vaccinia virus in tissue cultures was described. Gamma globulin, precipitated from rabbit antiserum, was conjugated to horseradish peroxidase with the aid of the bifunctional reagent FNPS. Vaccinia virus-infected cells on coverslips which were reacted with the enzyme-antibody complex and stained with diaminobenzidine, demonstrated the dark-brown cytoplasmic locatization of the antigen-antibody enzyme complex. Pretreatment with unlabeled antibody blocked the appearance of the cytoplasmic inclusions. The cell preparations were examined by ordinary light microscopy.
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