Abstract
Summary
A comparison of diluted rat blood and a semisynthetic perfusate was made with the use of isolated perfused rat livers. This was effected by determining the efficiency of ammonia metabolism in these media. Both perfusates allow a high degree of conversion of preformed ammonia to urea. Diluted rat blood, however, was far more efficient in the metabolism of nitrogen derived from oxidative deamination of glycine. A suggestion is made to investigators that synthetic perfusates be compared to freshly drawn blood within the proposed study parameter before designing research projects involving liver perfusion.
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