Abstract
Summary
Solubility fractionation of relatively crude TSH with ammonium sulfate was investigated as a means of purifying the hormone. The results obtained with three variations in solubility fractionation techniques using decreasing concentrations of ammonium sulfate demonstrated that each method yielded a 5- to 7-fold purification of the 0.49 to 0.84 U/mg starting material. Total recovery of TSH was 90-100%. Recoveries in the high potency fractions were 60-80% when TSH was extracted with ammonium sulfate or recovered by fractional precipitation and 25-35% when batch eluted from diethylaminoethyl (DEAE) cellulose. Solubility fractionation appears to be a simple and rapid method for the purification of TSH to 4-6 U/mg which may be adequate for most purposes. Highly purified TSH may then be obtained by standard column chromatography with DEAE-cellulose.
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