Abstract
Summary
Optimal conditions for thermal labilization (45°), in vitro, of isolated, hepatic, lysosomal suspensions were defined with respect to the release of acid phosphatase and β-glucuronidase, and the stability of these enzymes obtained by hyptonic disruption of lysosomes. Neither steroidal nor nonsteroidal anti-inflammatory drugs, in general, stabilized lysosomes with respect to release of both enzymes when the lysosomes were labilized thermally; some drugs enhanced labilization. Indomethacin (10-3 M) and phenylbutazone (10-3 M) inhibited β-glu-curonidase obtained from lysosomes. Results are discussed.
Addendum. Tanaka and Iizuka (22) have recently published on work similar to that reported herein. Our results are essentially in agreement with theirs and both conflict with an earlier report by Miller and Smith (12). In particular, our results confirm the labilization of lysosomes by acetylsalicylic acid in acidic sucrose medium and inhibition of “free” beta-glucuronidase activity by phenyl-butazone and indomethacin. We, however, did not incubate lysosomes with mild shaking as indicated in the above work (22). Agitation of the pastic tubes due to the swirling water of the bath plus thermal agitation at 45° seemed sufficient for our purposes.
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