Abstract
Summary
Highly purified (95% coagulable) canine fibrinogen was converted to fibrin by thrombin (8–10 NIH units) and the fibrin, collected on a siliconized glass rod, was subsequently dialyzed free of salts (distilled water), dehydrated (ethanol-ether 3v/2v) and dried to constant weight. Following solution of the dried fibrin in freshly prepared alkaline urea (40% urea in 0.2 N NaOH) the optical density of samples containing different amounts of fibrin was determined at 282 mμ in 1-cm quartz cuvettes. Least square analysis of the data reveals that the equation y = 1.743x + 0.015 best fits the data points with a correlation coefficient between optical density and fibrin concentration of 1.0 (0.9998). The extinction coefficient for a 0.1% solution of canine fibrin in alkaline urea is 1.743. This result is discussed with regard to extinction coefficients obtained from the study of other species.
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