Studies on Early-Appearing Interferon in Vitro I. Production of Endotoxin-Induced Interferon by Mouse Spleen Cells Cultured in Vitro

Abstract

Summary

Mouse spleen cells cultured in vitro produced at least two kinds of interferon (IF) in response to inoculation with E. coli endotoxin. The early-appearing IF showed its peak titer about 2 hr after the inoculation of inducer, while the late-appearing IF possessed its maximum titer at 6–8 hr. Their properties were generally similar to those of endotoxin-induced IF in mouse plasma or spleen in vivo. The production of endotoxin-induced IF in mouse plasma or spleen was in parallel with the appearance of lysosomal enzymes, β-glucuronidase and acid phosphatase. In the case of cultured spleen cells in vitro, such parallelism was not observed at all. The production of early-IF in vitro was not affected by treatment with actinomycin D (10μg/ml), but inhibited almost completely by puromycin (50 μg/ml). Both inhibitors caused almost complete inhibition of the production of late-IF.