Abstract
Summary
Proteins and LDH-isoenzymes were determined simultaneously in human serum and aqueous humor by an agar gel electrophoretic technique. The isoenzyme patterns of ocular tissues were obtained. Normal and uveitis cases were studied. The results showed that in uveitis, the protein concentration and isoenzyme activity in the aqueous humor increased; the protein pattern in the aqueous was similar to that of the serum indicating serum protein penetration into the aqueous, while the isoenzyme pattern of the aqueous showed an influence from both the serum and the ocular tissues. The combined determination of proteins and LDH-isoenzymes in serum and aqueous may be useful as a diagnostic aid, for permeability studies in the eye and for detection of metabolic changes of ocular tissues.
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