Hemolysis and Erythropoiesis V. The Utilization of Hemoglobin Iron by Bled Anemic Dogs ∗

Abstract

Summary

Two dogs with anemia induced by bleeding were injected intramuscularly with iron dextran to replace the iron lost through the bleedings. They were given intraperitoneally 1.5 ml/kg/day of a hemolysate containing 15 g/100 ml of ⁵⁹Fe-labeled hemoglobin. Seventeen and 24.7% of the injected heme-⁵⁹Fe was reincorporated in the RBC, which accounted for only 13.9 and 11.9% of the iron present in the hemoglobin gained following injections of hemolysate. These results indicate that the 60.7% increase in hemoglobin production after hemolysate administration observed in previous similar experiments (1), cannot be ascribed to availability of large amounts of heme iron.