Abstract
Summary
The quantitative analysis of prolactin was investigated by disc electrophoresis. A linear relationship was found between the amount of standard prolactin preparation used and the optical density of the band obtained, indicating that this method can be employed for the quantitative determination of prolactin. One-2 mg of fresh pituitary from mature female rats was sufficient for clear detection of prolactin. A high correlation was obtained between the optical density and the biological activity of the eluate of the band. Chronic administration of large doses of prolactin to rats decreased the optical density of the prolactin band to 59% of that in the control pituitary. It was also demonstrated that prolactin released in vitro from the anterior pituitary of rats could be detected by disc electrophoresis.
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