Abstract
Summary
A simple and reproducible technic for the preparation of active isolated rat adrenal cells has been described. Quartered glands are incubated at 37° with mechanical agitation in the presence of trypsin (0.25%). The dispersed cells are collected by centrifugation and suspended in Krebs-Ringer bicarbonate buffer to which has been added glucose (0.2%), albumin (3.0%) and trypsin inhibitor (2 mg/ml). Aliquots of the suspension are incubated at 37° for 2 hr. ACTH in concentrations of 10, 40, and 160 μU increases corticosterone production by the isolated cells. The regression line relating corticosterone production and log dose of ACTH is straight.
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