Hageman Factor Activation and Kinin Formation in Human Plasma Induced by Cellulose Sulfate Solutions ∗

Abstract

Summary

Cellulose sulfate was previously reported as an anticoagulant and as a substance that will induce kinin formation in normal human plasma in vitro. The present data confirm these findings but in addition indicate that at lesser concentrations it is a procoagulant as well. Further, cellulose sulfate will induce permeability-enhancing activity in normal human plasma. Cellulose sulfate does not induce the formation of kinins, or permeability-enhancing activity, nor does it enhance clotting when added to human plasma deficient in Hageman factor. Further the induction of permeability-enhancing activity in normal human plasma is blocked by the addition of rabbit anti-Hageman serum prior to the addition of cellulose sulfate. These data therefore indicate that cellulose sulfate, like ellagic acid, activates Hageman factor in normal human plasma in vitro. On the basis of this datum, it is suggested that the mechanism of kinin formation and kininogen depletion in dogs given cellulose sulfate (4) is the same as that proposed for ellagic acid, namely the activation of Hageman factor and sequential interaction of Hageman factor, PF/dil, kallikrein, and kininogen resulting in a consumptive depletion of kininogen (18).