Abstract
Summary
The appearance of the REF in the light mitochondrial and microsomal fractions of rat kidney was studied during a continuous 22.5 hr exposure to hypoxia. Within 15 min a depletion of the REF occurred in the microsomal fraction (105,000g) but ESF-generating activity was detectable at this time in the light mitochondrial fraction (21,000g). Beginning with 1 hr of hypoxia, REF activity reappeared in the microsomal but decreased in the light mitochondrial fraction. At 10 hrs, REF activity was again present in the light mitochondrial but not in the microsomal fraction. With further exposure, the REF was again evident in both fractions up to 22.5 hr. Plasma ESF values rose steadily from hours 2 to 10 followed by a decrease at hour 15 and a secondary rise at 22.5 hours. It is suggested that the REF is generated in the microsomal fraction from which the factor is transported to the peroxisomes contained within the light mitochondrial fraction.
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