Abstract
Summary
The nature of the insulin inhibitor associated with plasma albumin remains an enigma with possibilities ranging from the B chain of insulin to artifacts due to dialysis membranes or trichloracetic acid. Our observations that exogenous insulin or tolbutamide cause an increase in inhibitor levels in normal subjects suggested that insulin degradation (probably hepatic) could be involved (2). To test this possibility, normal rat serum was perfused through rat liver with and without added insulin. Subsequently atbumin was isolated and insulin inhibitory activity was measured. Inhibitor activity was significantly increased by liver perfusion. An apparent further increase occurred with added insulin.
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