Abstract
Summary
In normal, nephrectomized or adrenalectomized rats the injection of azamethonium (ganglionic blocking agent) increases the pressor effect of incubated plasma or its active fraction (VA) and also that produced by angiotensin and bradykinin. However the pressor activity of incubated plasma or VA given by a single injection or infusion is: (a) not modified by adrenalectomy, and (b) not inhibited by the previous injections of α or β adrenergic receptor blocking agents, such as phentolamine and propranolol, respectively. These results rule out that the pressor effect is produced by the liberation of catecholamines. Since phentolamine alone or after azamethonium reduces the pressor effects of angiotensin and markedly inhibits or abolishes the pressor action of bradykinin, the conclusion can be drawn that the pressor effect of plasma and VA is not due to the release of angiotensin or bradykinin. The results of the gel filtration and electrophoresis of incubated plasma are in agreement with the view that VA is an albumin or an albumin bound molecule.
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