Prolonged Culture of Human Leukocytes in an Isolated Environment

Abstract

Conclusions

Cells capable of continuous propagation in vitro were cultured from peripheral blood of normal individuals under conditions of complete isolation from other cell lines. A lag phase of 7 weeks preceded rapid multiplication. Cultivation of these cells is apparently not dependent on the presence of a herpes-like virus similar to the leukovirus found in Burkitt's lymphoma. The line has been cultured continuously for 1 year.