Labeling of Marrow Cells of Vitamin E-Deficient Monkeys by 3 H-Precursors of Nucleic Acids and Protein ∗

Abstract

Summary

The incorporation of ³H-labeled thymidine, deoxyuridine, uridine, and l-leucine into bone marrow cells of vitamin E-deficient and control monkeys was measured in autoradiographic studies. During the anemic of vitamin E-deficiency there was an increase in the grain count per labeled cell of most cell types in the erythroid and myeloid series, while the percentage of ³H-labeled cells was not altered from control values. Multinucleated erythroid cells in the bone marrow of vitamin E-deficient monkeys were not labeled by thymidine-³H. These findings are evidence that active erythropoiesis associated with premature death of erythroid cells occurs in the hypercellular bone marrow of anemic vitamin E- deficient monkeys.