Abstract
As is well known, it is possible, by means of salting out with appropriate percentages of ammonium sulphate or sodium chloride, to almost entirely separate the albumin from the globulin and the pseudo-globulin of serum. This has been done in the preparation of diphtheria antitoxin, where it has been found that the antitoxin is closely associated with the pseudo-globulin fraction.
A similar procedure was carried out to determine the association of the coagulative principles of the serum. It was found that in human plasma as well as in horse plasma, these substances are linked with the euglobulin fraction. If these three proteid fractions of the serum are separated and dissolved in normal salt solution and added to plasma (with the addition of a small amount of calcium) the euglobulin will markedly hasten coagulation, whereas the two other fractions will have either no effect or a slightly inhibitory action. It is possible in this way to prepare a refined serum containing only 1/2 to 1 per cent. proteid, that is to say, one tenth to one fifth the quantity present in normal serum, but possessing an equal potency as regards coagulation. This preparation may be passed through a Berkefeld filter so as to be rendered sterile.
This euglobulin would seem to be of value for subcutaneous or intravenous use in hemorrhage, particularly on account of its small quantity of proteid.
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