Abstract
Summary
Homogenates prepared from normal rabbit kidneys were found to contain a strong inhibitor of human erythropoietin obtained from human urine or from human plasma. The kinetics of the inactivation suggests it to be a nonenzymatic process. Dialysis against water or EDTA saline did not change the activity of the inhibitor and heating to 100°C did not completely abolish its activity. The addition of normal plasma reduced the inhibitory activity of the renal homogenate, a reduction that was directly proportional to the amount of normal plasma added and was observed both with urinary erythropoietin and with plasma erythropoietin. Homogenates prepared from normal rabbit spleens and livers were also found to contain an inhibitory principle that was modified by the addition of normal plasma. Homogenates from normal rabbit bone marrow were almost devoid of inhibitory activity.
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