Abstract
Summary
A replicative type DNA polymerase has been partially purified from the nonhistone chromosomal proteins of Walker 256 carcinosarcoma. The enzymic polymerization of deoxyribotides into DNA-like product depends on the presence of a complete supplement of all four deoxyriboside-5′-triphosphates and native DNA as template. The enzyme requires Mg2+ for its optimal reaction and is inhibited by Na+ and K+. The DNA in isolated chromatin exhibits 40% of its templating activity. The tumor chromosomal DNA polymerase shows only marginal activity with reconstituted DNA-histone as template.
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