Abstract
Summary
A sensitive and rapid isotopic microassay method for cholinesterase determination in biological fluid and tissue has been described. The method employed acetyl-1-14C choline as a specific substrate. Free acetic acid labeled with 14C liberated enzymatically is selectively extracted in toluene isoamyl alcohol and measured by simple counting technique. The potential of the method is illustrated by determination of the level of the enzyme in brain homogenates and its subcellular fractions as well as in human whole blood. The method has the advantage that it can be applied to samples from 10 μg to 2 mg at constant pH. A wide range of technique is demonstrated.
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