Abstract
Summary
The in vitro and in vivo culture methods for consistently obtaining mitotic divisions suitable for chromosome analysis from peripheral blood of various mouse strains have been described. In the in vitro culture system pokeweed was used as the mitogenic agent. The DNA-synthesis and mitotic activity commenced much earlier than in the diffusion chamber cultures in which the allogeneic or heterologous environment provided the mitogenic stimulus. In the latter system cells could be maintained for at least 7 days. Mitoses were still frequent at this time.
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