Inhibition of Triglyceride Synthesis in Everted Intestinal Sacs ∗

Abstract

Summary

Optimal conditions for synthesis of triglyceride from oleic acid-1-¹⁴C in everted intestinal sacs were determined. Using several experimental conditions, it was found that 2-ethyl-n-caproate inhibited oleic acid incorporation into triglyceride by 25-50%. When inhibition of triglyceride synthesis occurred, there was a concomitant increase in mucosal unesterified fatty acids but no increase in the phospholipid, mono- or diglyceride fractions. The inhibition of glyceride synthesis in the intestinal mucosa by 2-ethyl-n-caproate appears to be at the level of long-chain fatty acid activation.