Effect of Growth Hormone and Glucose on 14 C Incorporation by the Perfused Dog Pancreas ∗

Abstract

Summary

The isolated, recirculated, perfused dog pancreas has been used to study the effect of elevated glucose concentrations and GH on the incorporation of ¹⁴C labeled amino acids into the insulin containing fraction. The greatest incorporation occurred at the low glucose level. High glucose levels alone and 1 or 3 units of GH at low or high glucose levels caused a reduction of ¹⁴C amino acid incorporation into the IRI containing fraction below that seen with low glucose levels alone. Pretreatment of the pancreas donors with GH restored the ¹⁴C incorporation to that seen with low glucose alone. The results are discussed.