Abstract
Summary
A method for fractionation of rosette-forming cells has been described. Incubation mixtures of SRBC with antibody producing mouse spleen cells in which rosettes had been formed were layered on BPA density gradients for centrifugation, with resulting enrichment of rosettes relative to the original cell suspension. Certain fractions consisted of up to 40% rosette-forming cells. The possibility that rosettes were formed passively under the conditions of this study was ruled out by a series of experiments. Production of rosettes did not result from incubation of normal spleen cells with mouse anti-SRBC serum or with medium in which antibody producing suspensions of cells had been incubated. The production of rosettes could be suppressed by prior incubation of the cells with iodoacetate or with antileukocyte or antispleen cell serum produced in allogeneic animals.
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