Abstract
Summary
The infectivity of RS virus was enhanced by the addition of DEAE dextran to the virus inoculum or in the mixture. The maximal effect (2- to 5-fold) was obtained with 30 μg/ml in HEp-2 cells and 10 μg/ml in AGMK cells in the virus inoculum. The enhancing action of this polyion with RS virus was apparently exerted at the early stages of virus-cell interaction and involved direct complexing with virus particles. Although protamine sulfate enhanced the infectivity in the mixture as much as DEAE dextran, it inhibited the infectivity to 50% at a concentration of 60 μg/ml after a slight increase with 5-10 μg/ml in the virus inoculum. Dextran sulfate and heparin decreased markedly the infectivity of the virus (28 and 18%) with a concentration as low as 5 μg/ml in the virus inoculum in HEp-2 cells, and also showed an inhibitory effect in the mixture.
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