Studies on the Nature of the Synalbumin Insulin Antagonist ∗

Abstract

Summary

“Albumin” extracted from human plasma by the method of Debro and dialyzed through boiled dialysis membranes for 7 days has been shown to antagonize the effect of insulin on the isolated rat hemidiaphragm. The antagonist has been separated from “albumin” by ion-exchange chromatography. This nonantagonistic albumin did not become antagonistic when redialyzed through boiled or unboiled dialysis membranes. Nonantagonistic “albumin” did become antagonistic when reextracted from an aqueous solution by the Debro procedure and dialyzed through either boiled or unboiled dialysis membranes. An aqueous extract from the dialysis membranes did not exhibit significant insulin antagonism at concentrations as high as 1000 μg/ml.