Density Gradient Ultracentrifugation of Rubella Virus

Abstract

Summary

The buoyant density of rubella virus HA was determined to be 1.23 in Cs₂SO_4. A density of 1.33 was obtained in CsCl. Rubella virus HA, either Tween-80. ether-treated or untreated, and infectivity could not be separated on sucrose by rate zonal or density gradient techniques. These activities also could not be separated on CsCl and Cs₂SO₄ density gradients. The banding density of rubella virus HA preparations was unaffected by the type of cell culture used for antigen production; neither the passage level nor the strain of virus altered the point at which rubella virus HA banded in CsCl.