Abstract
Summary
Two reliable methods for plaque titration of herpes simplex in antibody-free liquid culture medium have been described. Some advantages over plaque titrations with solid or antibody-containing overlays are elimination of inhibitory effects of agar and antiserum, greater choice in type of culture vessel which allows for saving of culture materials, technician's time, and incubator space, optional elimination of carbon dioxide incubators, greater range of magnification, and more flexibility in manipulation of cultures.
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