Growth and Glycolysis in Two Cell Lines Derived from Embryos of Salmonid Fish ∗

Abstract

Summary

Measurements of growth and glycolysis in 2 established heteroploid fibro blast-like cell lines derived from embryos of coho salmon and steelhead trout, growing at 23°, have been compared with similar measurements in cultures of a strain of diploid cells derived from human embryos, growing at 35°. The growth rate of the coho salmon cells was nearly as great as that of the human cell strain. The rates of glucose utilization and lactic acid production by the salmonid cell lines were lower than those of the human cells. The proportion of glucose used which appeared as lactic acid in the culture medium was frequently lower in the fish cell cultures. The data obtained seemed to indicate that the process of glycolysis in these lines of salmonid fish cells proceeds in a manner very similar to that in mammalian cell cultures and that differences which exist are chiefly quantitative in nature.