Abstract
Summary
Measuring the loss of histidine-tritium from cellular protein provides a sensitive assay for in vivo protein photooxidation. The relative rates of tritium loss from the various protein amino acids in vivo correlate with the relative rates of photodamage known to occur to them in vitro: histidine > tryptophan > tyrosine > arginine and leucine. Moreover, the degree of loss of histidine-tritium during in vivo photodynamic activity correlates with the degree of photokilling.
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