Abstract
Summary
Butanol extracts of normal human and rat urine contained two types of substances, distinct from each other and unrelated to PTH, which interfered with the bio-assay of PTH in urine. One caused hypercalcemia, the other hyperphosphaturia in PTX rats. The latter material was dialyzable and equilibrated between 20% acetic and n-butanol with a distribution coefficient (K) about 10 times lower than that of PTH. The “hypercalcemic” material was nondialyzable and its K about 5 times lower than that of PTH. Thus, both materials could be separated by CCD from PTH added to the urine before extraction. From 100 ml of normal (human or rat) urine, no PTH activity could be detected in fractions expected to contain PTH in its known form.
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