Abstract
Summary
Intradermal injection of either actinomycin D or puromycin locally over 1 side of the pigeon crop-sac inhibited the tissue's response to systemically administered prolactin. RNA extracted from prolactin-stimulated crop-sac mucosal tissue had a high degree of crop-sac stimulating activity when injected intradermally over the crop-sac. In contrast, similar RNA fractions extracted from pigeon liver tissue or from liver to which prolactin was added prior to extraction were devoid of this activity. Hydrolysis of the crop-sac RNA by RNase completely eliminated its crop-sac stimulating capacity. These results indicate that RNA and protein synthesis is prerequisite for the expression of the crop-sac response to prolactin.
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