Abstract
Summary
Infection of chick embryo cell monolayers in high multiplicity with strain MC29 leukosis virus in conventional tissue culture induces rapid, massive morphological conversion within 4 to 7 days. Treatment of the cells in appropriately lower inoculation multiplicities results in the occurrence of infectious centers distinguished as foci of converted cells. When the infected cells are overlaid with agar, the number of foci occurring in 7 days is closely proportional to virus dose. This relationship is applicable to bioassay of strain MC29 leukosis virus with reproducibility and precision of results compatible with practical quantitative studies on the agent. The bioassay procedure and the rapid massive culture conversion make available a unique avian virus-tumor system for studies on cell-virus interrelationships in the induction of neoplasia.
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