A DEAE-Cellulose Chromatographic Separation of Co 57 Vitamin B 12 Binders in Normal Human Serum. ∗

Abstract

Summary

A method was described for the separation of vit B₁₂ binders in normal serum on a 15 × 4 cm DEAE-cellulose column by stepwise elution with 0.005 M pH 8.0, 0.0175 M pH 6.3, 0.04 M pH 5.9, 0.1 M pH 5.8 and 0.4 M pH 5.2 sodium phosphate buffers. Two radioactive peaks appeared when 300 μμg Co⁵⁷ B₁₂ per ml of serum had been added in vitro. With the addition of 2,000 μμ/ml of Co⁵⁷ B₁₂ to the serum, 5 radioactive peaks appeared.