DNA Biosynthesis in Monkey Kidney Cells Infected With PARA (SV40)-Adenoviruses. ∗

Abstract

Summary

Studies conducted to follow biosynthesis of deoxyribonucleic acid (DNA) in cultures of African green monkey kidney (GMK) cells inoculated with PARA (SV40)-adenoviruses 2, 7, and 12 showed an initial depression in incorporation of H³-thymidine into the DNA. Levels of DNA started to increase after 15 hours, reaching a maximum in the virus inoculated cultures 24 hours post-infection. Extraction of the DNA from infected cells, followed by their identification by isopycnic analysis in CsCl gradients, revealed the presence of virus DNA with a density corresponding to the adenovirus serotype used. DNA samples from GMK cultures inoculated with PARA-adenovirus 7 or its transcapsidants, PARA-adenovirus types 2 and 12, yielded bands with peak densities of 1.710 g/ml [adenovirus type 7 DNA with 51 moles % guanine + cytosine (GC)], 1.715 g/ml (adenovirus type 2 DNA with 56 moles % GC), or 1.706 g/ml (adenovirus type 12 DNA with 47 moles % GC), respectively as well as a band at a density of 1.699 g/ml (GMK DNA with 40 moles % GC). A separate band that might have been distinctive for the DNA of the PARA particle was not detected.