Abstract
Summary
The effect of a single dose of TSH on the incorporation in vivo of 14C labeled amino acids into thyroid proteins of the rat have been studied. Soluble thyroid proteins were analyzed by sucrose density-gradient ultracentrifugation. TSH, injected 2 to 4 hours before pulse-labeling, increased the total amount of 14C in protein and accelerated the appearance of label in 19 S thyroglobulin. When TSH was given within one hour of labeling or two hours afterwards, total 14C incorporation was unaffected, but the label appeared in mature (18-19 S) thyroglobulin at the expense of label in an immature (15-16 S) precursor protein. The results suggest that TSH produces an early stimulation of several phases of thyroglobulin biosynthesis.
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