Abstract
Summary
The kinetics of proline and hydroxyproline and their respective incorporation into dentine collagen during rapid dentinogenesis has been reported. The incorporation of proline into collagen and the subsequent hydroxylation of this imino acid takes place as an intracellular event, demonstrated by the rapid rise in the specific activity of hydroxyproline in the neutral salt collagen fraction and the intracellular localization of the grain density. Four hours after administration of proline C14, the biosynthesis of collagen and its export from the odontoblast into the extracellular organic matrix was completed. Both proline and hydroxyproline exhibit similar specific activities in the insoluble collagen fractions throughout the experimental period. The developing rodent molar was found to be an excellent experimental model for the study of rapid collagen synthesis and its role in the formation of the dentine organic matrix.
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