Ribonucleic Acid Synthesis and Nuclear Ribonucleic Acid Polymerase Activity in Livers of Mice Infected With Mouse Hepatitis Virus (MHV-3). ∗

Abstract

Summary

RNA polymerase activity, measured in vitro in the nuclear fraction of livers from mice infected with MHV-3 virus, was decreased 23% and 50% at 24 and 36 hours respectively after inoculation of the virus. The in vivo incorporation of orotate radioactivity in the nuclear RNA of liver cells showed no change from the normal during the first 24 hours after infection when viral multiplication had already reached its peak level, but a significant increase was evident at 36 hours. The cytoplasmic RNA of liver cells showed no change in in vivo incorporation of orotate radioactivity even at 36 hours after infection when the hepatocellular lesions were already marked.